Cell Proliferation ELISA, BrdU

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- BrdU solution (10 μM) was added during 24 h at 37°C	- Absorbance was measured at 370 nm (reference wavelength 492 nm).

Protocol tips
- BrdU solution (10 μM) was added during 24 h at 37°C
Downstream tips
- Absorbance was measured at 370 nm (reference wavelength 492 nm).

Publication protocol

The BrdU Cell Proliferation ELISA kit (Roche) has been used to measure cell proliferation according to manufacturer’s protocol. HepG2 cells were seeded at 2 500 cells per well into 96-well plates. 48 h after transfection, the BrdU solution (10 μM) was added during 24 h at 37°C. Absorbance was measured at 370 nm (reference wavelength 492 nm).

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