BrdU Cell Proliferation Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Cells are seeded and cultured in a medium containing 10% fetal bovine serum . - Cells were seeded and incubated for 72 h prior to addition of the BrdU solution.

Upstream tips
- Cells are seeded and cultured in a medium containing 10% fetal bovine serum . - Cells were seeded and incubated for 72 h prior to addition of the BrdU solution.

Publication protocol

Cells were seeded at a density of 50 000 cells per well in a six-well plate and maintained in media containing 10% fetal bovine serum. At the end of the experiment, cell number was determined using trypan blue exclusion. Cell proliferation assays were carried out using the bromodeoxyuridine (BrdU) cell proliferation assay kit (BioVision, Milpitas, CA, USA) according to the manufacturer's protocol. Briefly, 10 000 cells/well were seeded into a 96-well plate and incubated for 72 h prior to addition of the BrdU solution. BrdU incorporation was measured by absorbance at 450 nm. For clonogenic assay, cells were seeded onto 60 mm culture dishes at the density of 1000 cells/well. Cultures were maintained for 9–12 days in complete culture media until colonies appeared. The colonies were fixed with 4% paraformaldehyde and stained with 0.5% crystal violet. The plates were photographed and the numbers of visible colonies were counted.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell cytotoxicity / Proliferation assay cell type - SKOV-3 using BrdU Cell Proliferation Assay Kit from Biovision.

Manufacturer protocol

Download the product protocol from Biovision for BrdU Cell Proliferation Assay Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell cytotoxicity / Proliferation assay cell type - SKOV-3 using BrdU Cell Proliferation Assay Kit from Biovision. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.