Cell Proliferation Kit I (MTT)

Cell cytotoxicity / Proliferation assay cell type - A549

Experiment
Cell cytotoxicity / Proliferation assay cell type - A549
Product
Cell Proliferation Kit I (MTT) from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
- Seeded cells in a 6 well plate were treated with medium containing 10% FBS for 2 weeks

Publication protocol

The Cell Proliferation Reagent Kit I (MTT; Roche Applied Science) was used to test the cell viability. A549 and PC-9 cells plated in 96-well plates (3,000 cells/well) were transfected with si-PVT1. We estimated the cell viability every 24 hours according to the manufacturer's protocol. The MTT experiments were conducted in quadruplicate. For colony formation assay, transfected cells (n = 1200) were placed in 6-well plates and maintained in proper medium containing 10% FBS for 2 weeks. The medium was replaced every 4 days, and visible colonies were counted after 2 weeks.

Full paper   Login or join for free to view the full paper.

Reviews

Cell Proliferation Kit I (MTT) from Sigma-Aldrich has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell cytotoxicity / Proliferation assay cell type - A549 using Cell Proliferation Kit I (MTT) from Sigma-Aldrich.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Cell Proliferation Kit I (MTT) below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell cytotoxicity / Proliferation assay cell type - A549 using Cell Proliferation Kit I (MTT) from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms