Cell Proliferation ELISA, BrdU

Cell cytotoxicity / Proliferation assay cell type - FADU

Experiment
Cell cytotoxicity / Proliferation assay cell type - FADU
Product
Cell Proliferation ELISA, BrdU from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
- 3,000 cells/well were seeded in a 96 well plate
Protocol tips
- Cells were labelled with BrdU for 4 h.

Publication protocol

For the cell proliferation assay, a cell proliferation ELISA kit for the thymidine analog 5-bromo-2′-deoxyuridine (BrdU; Roche Diagnostics, Mannheim, Germany) was used as per the manufacturer’s instructions. In brief, cells were seeded in 96-well plates and the initial cell number was adjusted to 3,000/well. Following drug treatment, the cells were labeled with BrdU for 4 h. Subsequently, anti-BrdU-POD Fab fragments and substrate were added to the medium. The optical density (OD) was determined at 405 nm using an iMark Microplate Reader. The results were normalized to the control (the group treated with vehicle).

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Paper title
Inhibition of cancer cell proliferation by midazolam by targeting transient receptor potential melastatin 7
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Cell Proliferation ELISA, BrdU below.

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