Pierce™ LDH Cytotoxicity Assay Kit

Cell cytotoxicity / Proliferation assay cell type - HEK 293

Experiment
Cell cytotoxicity / Proliferation assay cell type - HEK 293
Product
Pierce™ LDH Cytotoxicity Assay Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Downstream tips
- If there is a high variability of
absorbance well-to-well , Centrifuge the plate for a longer time or at a higher speed.

Publication protocol

Supernatants from siRNA-transfected HEK293 cells were collected and assayed in triplicate
using a PierceTM lactate dehydrogenase (LDH) cytotoxicity assay kit (Thermo) according to the
manufacturer’s instruction. The assay was measured by 490nm absorbance with a microplate
spectrophotometer (Bio-rad). Percent LDH release was calculated after background subtraction
with blank wells.

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Papers

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Paper title
Drebrin restricts rotavirus entry by inhibiting dynamin-mediated endocytosis
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Pierce™ LDH Cytotoxicity Assay Kit below.

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