In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based

Cell cytotoxicity / Proliferation assay cell type - THP-1

Experiment
Cell cytotoxicity / Proliferation assay cell type - THP-1
Product
In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Downstream tips
- colorimetric absorbance was measured at 450 nm.

- Absorbance was determined at 620 nm

Publication protocol

The THP-1 cells were treated with various extracts or DMSO concentrations. Cells in the positive control wells were treated with 1% Triton X-100 solution, and negative control wells cells were incubated in culture media alone. Blank wells contained the corresponding extract concentrations or Triton X-100 solution or media without cells. The lactate dehydrogenase (LDH) based in vitro toxicology assay kit was used to assay for cytotoxicity following the manufacturer instructions. The assay measures membrane integrity as a function of the amount of cytoplasmic LDH released into the medium. LDH reduces NAD into NADH, which is utilized in the reduction of a tetrazolium dye to colored formazan. The amount of formazan which is proportional to the amount of LDH release from dead cells was measured colorimetrically at 450 nm. Absorbance for background correction was determined at 620 nm.

The percentage of cell viability was calculated as follows: % Cell viability = 100 –% cell cytotoxicity. The % cell cytotoxicity = 100 X (experimental well absorbance –negative control well absorbance) / (positive control well absorbance –negative control well absorbance). All calculations were performed after background absorbance correction and blank absorbance subtraction

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Paper title
inhibition of human leukemia THP-1 cells by L. and L. extracts
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based below.

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