Cytotoxicity Detection KitPLUS (LDH)

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	- Cell supernantant was assayed after 24h of treatement with reagents.

Protocol tips
- Cell supernantant was assayed after 24h of treatement with reagents.

Publication protocol

To evaluate damaged HT-22 cells in these experiments, the release of LDH was quantitated in the culture medium using the Cytotoxicity Detection Kitplus (Roche Applied Bioscience, Indianapolis, IN, USA) following the manufacturer’s protocol. Following 24 h of the indicated cell treatment, medium was collected for analysis of LDH. After subtracting the background values in the medium, the percentage cytotoxicity was calculated with the following equation: LDH release (% of Max) = 100 × (experimental value − low control)/(high control − low control). Experimental value, LDH values in the experimental groups; Low control, LDH values in the untreated normal cells; High control, the maximum releasable LDH values in the untreated normal cells.

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