Cell Proliferation Kit II (XTT)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 104 cells/well were seeded on a 96-well plate. - Cells were treated with ester, amino, cis-TMS and trans–TMS in a concentration ranging from 7.8 μM to 1000 μM and incubated for an additional 24 h		- Total absorbance was measured at 492

Upstream tips
- 104 cells/well were seeded on a 96-well plate. - Cells were treated with ester, amino, cis-TMS and trans–TMS in a concentration ranging from 7.8 μM to 1000 μM and incubated for an additional 24 h
Downstream tips
- Total absorbance was measured at 492

Publication protocol

To determine cell viability, 104 cells/well were seeded on a 96-well plate. After 24 h the cells were treated with ester, amino, cis-TMS and trans–TMS in a concentration ranging from 7.8 μM to 1000 μM and incubated for an additional 24 h. The plates were washed twice in phosphate buffer saline (PBS 1X) and incubated for 4 h at 37 °C with DMEM without phenol red and supplemented with the reagents of the Cell Proliferation Kit (XTT) as recommended by the manufacturer. Total absorbance was measured at 492 and 690 nm (reference) using a microplate reader (ASYS, Eugendorf, Salzburg, Austria). Results of total absorbance were considered directly proportional to the number of viable cells as a percentage of the negative control (100% of cell viability).

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