TiterTACS™ Colorimetric Apoptosis Detection Kit

TUNEL assay cell type - PC-3 human prostate cancer

Experiment
TUNEL assay cell type - PC-3 human prostate cancer
Product
TiterTACS™ Colorimetric Apoptosis Detection Kit from Trevigen
Manufacturer
Trevigen

Protocol tips

Protocol tips
Add Proteinase K solution and incubate for 15 minutes at room temperature.

Add Labeling Reaction mix and incubate at 37°C for 1 hour.

Add TACS-Sapphire at room temperature..

Publication protocol

The co-culture setup for this assay was identical to the CD107a assay above. 1–3 days after co-culture in 96-well plates, DNA fragmentation was assessed using the TiterTACS Colorimetric Apoptosis Detection (TUNEL) Kit from Trevigen, according to manufacturer recommendations.

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Discussion

Discussion

2 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

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Manufacturer protocol

Download the product protocol from Trevigen for TiterTACS™ Colorimetric Apoptosis Detection Kit below.

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