ApopTag® Peroxidase In Situ Apoptosis Detection Kit

TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells

Experiment
TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells
Product
ApopTag® Peroxidase In Situ Apoptosis Detection Kit from Millipore
Manufacturer
Millipore

Protocol tips

Protocol tips
Cells were treated with 20 mg/ml proteinase K for 15 min at room temperature after after deparaffinization.

- hydrogen peroxide is applied for 5 min at room temperature.

Publication protocol

5-FU-induced cell death was investigated on the day 14 using the terminal deoxynucleotidyltransferase (TdT)-mediated dUTP nick end labelling (TUNEL) method (ApopTag®, no. S7101; Merck Millipore, Darmstadt, Germany). Briefly, after deparaffinizing, the samples were rehydrated and incubated with 20 mg/ml proteinase K for 15 min at room temperature. Endogenous peroxidases were blocked by treatment with 3% (v/v) hydrogen peroxide in phosphate-buffered saline (PBS) for 5 min at room temperature. After washing, the sections were then incubated in a humidified chamber at 37°C for 1 h with TdT buffer containing TdT enzyme and reaction buffer. Specimens were incubated for 10 min at room temperature with a stop/wash buffer and then incubated in a humidified chamber for 30 min with anti-digoxigenin peroxidase conjugate at room temperature. After a series of PBS washes, the slides were covered with peroxidase substrate for color development and then washed in three changes of dH2O and counterstained in 0.5% (w/v) methyl green for 10 min at room temperature. The TUNEL-positive cells were counted (10 fields/slide; magnification, ×1,000) for statistical comparisons.

Full paper   Login or join for free to view the full paper.

Reviews

ApopTag® Peroxidase In Situ Apoptosis Detection Kit from Millipore has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

4 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells using ApopTag® Peroxidase In Situ Apoptosis Detection Kit from Millipore.

Paper title
Protective effect of Bunge on 5-fluorouracil-induced oral mucositis
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Millipore for ApopTag® Peroxidase In Situ Apoptosis Detection Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells using ApopTag® Peroxidase In Situ Apoptosis Detection Kit from Millipore. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms