DeadEnd™ Fluorometric TUNEL System

TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer

Experiment
TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer
Product
DeadEnd™ Fluorometric TUNEL System from Promega
Manufacturer
Promega

Protocol tips

Downstream tips
- When there was a little or poor staining, optimise permeabilization step
by adjusting incubation time with permeabilization agent.


- When there is a loss of tissue from slides, decrease
Proteinase K incubation time.

Publication protocol

Clinical-grade gefitinib was kindly provided by AstraZeneca. Cisplatin was purchased from Sigma-Aldrich. The anti-phospho-Akt, anti-Akt, anti-phospho ERK, anti-ERK, anti-phospho EGFR, anti-EGFR, anti-HER2, and anti-PARP antibodies were obtained from Cell Signaling. The anti-HER3 antibody was purchased from Upstate Cell Signaling Solutions. The terminal deoxynucleotidyltransferase-mediated dUTP nick end-labeling (TUNEL) kit (DeadEndTM Fluorometric TUNEL System®) was obtained from Promega.

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Reviews

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Discussion

Discussion

4 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer using DeadEnd™ Fluorometric TUNEL System from Promega.

Paper title
Gefitinib (ZD1839) increases the efficacy of cisplatin in ovarian cancer cells.
Full paper
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Manufacturer protocol

Download the product protocol from Promega for DeadEnd™ Fluorometric TUNEL System below.

Download PDF Download manufacturer protocol

Videos

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