ApopTag Fluorescein in Situ Apoptosis Detection Kit

TUNEL assay cell type - HEK293 human embryonic kidney cells

Experiment
TUNEL assay cell type - HEK293 human embryonic kidney cells
Product
ApopTag Fluorescein in Situ Apoptosis Detection Kit from Millipore
Manufacturer
Millipore

Protocol tips

Protocol tips
- Fix 5 x 10^7 cells/mL cells in 1% PFA in PBS, pH 7.4

- After applying Apply Equilibration Buffer and Working Strength TdT Enzyme , incubate at 37C for 1 hour.

Publication protocol

Tunel staining was performed with the use of an ApopTag Fluorescein Direct in Situ Apoptosis Detection kit (Chemicon, UK) according to the manufacturer’s instructions. Images were visualized by specific green fluorescence and nuclei by 4'-6-diamidino-2 phenylindole (DAPI) (nuclear counterstain). The TUNEL positives cells were examined with a microscope (Nikon TE-2000 U) and images were acquired with a digital camera (Nikon).

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Discussion

Discussion

2 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - HEK293 human embryonic kidney cells using ApopTag Fluorescein in Situ Apoptosis Detection Kit from Millipore.

Paper title
β1-Adrenergic Receptor and Sphingosine-1-Phosphate Receptor 1 Reciprocal Down-Regulation Influences Cardiac Hypertrophic Response and Progression Toward Heart Failure: Protective Role of S1PR1 Cardiac Gene Therapy
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Manufacturer protocol

Download the product protocol from Millipore for ApopTag Fluorescein in Situ Apoptosis Detection Kit below.

Download PDF Download manufacturer protocol

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