Gibco™Advanced DMEM/F-12

3D Cell Culture Media Mouse liver organoids

Experiment
3D Cell Culture Media Mouse liver organoids
Product
Gibco™Advanced DMEM/F-12 from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Seeding of ciliary ducts or sorted cells

Publication protocol

Liver organoid culture: Biliary ducts or sorted Lgr5-LacZ+ cells were isolated, mixed with Matrigel (BD Bioscience) and cultured as described in ref. 2. Medium composition was AdDMEM/F12 (Invitrogen) supplemented with B27 and N2 (Invitrogen), N-Acetylcysteine (1.25 μM, Sigma), gastrin (10 nM, Sigma), EGF (50 ng/ml, Peprotech), 10% RSPO1 Conditioned Medium (kindly provided by Calvin Kuo), FGF10 (100 ng/ml, Peprotech), nicotinamide (10 mM, Sigma) and HGF (50 ng/ml, Peprotech). Detailed protocols are provided in Supplementary Methods.

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™Advanced DMEM/F-12 below.

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