MEBMTM Mammary Epithelial Cell Growth Basal Medium

Protocol tips

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	Seed of cells at several densities (10, 50, 100, 1000 cells per well) Use of 96-well ultra-low attachment plates Count of >50μm mammospheres

Protocol tips
Seed of cells at several densities (10, 50, 100, 1000 cells per well) Use of 96-well ultra-low attachment plates Count of >50μm mammospheres

Publication protocol

Cells were seeded at several densities (10, 50, 100, 1000 cells per well) in 96-well ultra-low attachment plates (Corning, #3474) with MEBM Basal Medium (Lonza, CC-3151) containing 2% B-27 (Invitrogen, 17504-044), 20 ng/mL EGF (Sigma, E9644), 4 μg/mL insulin (Novo Nordisk, # 3525909), and 2 μg/mL hydrocortisone (Sigma, H0135). Mammospheres were cultured for 1-2 weeks, with image collection approximately every three days starting at day 8. Whole-well images were taken with the IncuCyte ZOOM System (Essen Bioscience) using a 4X phase contrast objective. Mammosphere diameter and the number of mammospheres >50μm were determined by image analysis using Fiji software (Fiji).

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Manufacturer protocol

Download the product protocol from Lonza for MEBMTM Mammary Epithelial Cell Growth Basal Medium below.

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