MammoCult™ Human Medium Kit

3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres

Experiment
3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres
Product
MammoCult™ Human Medium Kit from STEMCELL technologies
Manufacturer
STEMCELL technologies

Protocol tips

Protocol tips
Seed at a density of 5,000 cells/mL
Use 6-well ultra-low-attachment plates
Count mammospheres ≥50 μm in diameter

Publication protocol

Cultured cells were trypsinized, whereas tumor tissues were minced, digested with 1 mg/mL of type 1 collagenase (Sigma-Aldrich) at 37°C for 30 min, and filtered through a 70-μm cell strainer. The number of live cells was determined using trypan blue staining. Single-cell suspensions were seeded in 6-well ultra-low-attachment plates (Corning) at a density of 5,000 cells/mL in complete MammoCult Medium (STEMCELL Technologies). Mammosphere cultures were photographed 7 days later using a phase contrast microscope (Olympus), and mammospheres ≥50 μm in diameter were counted using ImageJ software (NIH). Primary mammospheres were collected by centrifugation and dissociated using trypsin, and single-cell suspensions were re-seeded at a density of 2,500 cells/mL for the secondary mammosphere assay.

Full paper   Login or join for free to view the full paper.

Reviews

MammoCult™ Human Medium Kit from STEMCELL technologies has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing 3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres using MammoCult™ Human Medium Kit from STEMCELL technologies.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from STEMCELL technologies for MammoCult™ Human Medium Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing 3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres using MammoCult™ Human Medium Kit from STEMCELL technologies. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms