MEGMTM Mammary Epithelial Cell Growth Medium BulletKitTM

3D Cell Culture Media BT-549 cells-Mammospheres

Experiment
3D Cell Culture Media BT-549 cells-Mammospheres
Product
MEGMTM Mammary Epithelial Cell Growth Medium BulletKitTM from Lonza
Manufacturer
Lonza

Protocol tips

Protocol tips
Seed BT549 cells at a density of 2500 cells/wel/
Use of 6-well ultra-low attachment plates
Count mammospheres with diameters of ≥100 μm

Publication protocol

BT549 and MDA-MB-231 cells, when seeded in ultra-low attachment plates (Corning, Corning, NY) in serum-free media form non-adherent spheroids termed mammospheres, with the ability to self-renew (Fillmore and Kuperwasser, 2008). To examine the effects of various treatments on mammosphere formation, BT549 and MDA-MB-231 cells were seeded in 6-well ultra-low attachment plates in plating medium (MEGM) with and without added treatments at a density of 2500 cells per well. 24h after, chemotherapeutic agents were added into plate. The appearance of spheres was evaluated after 7 (BT-549) or 10-12 (MDA-MB-231) days. Mammospheres with diameters of ≥100 μm (BT-549) and ≥50 μm (MDA-MB-231) were manually counted under microscope.

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Papers

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Manufacturer protocol

Download the product protocol from Lonza for MEGMTM Mammary Epithelial Cell Growth Medium BulletKitTM below.

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