NucleoSpin® Soil

DNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus

Experiment
DNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus
Product
NucleoSpin® Soil from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Upstream tips
Buffers A3 and AW contain guanidine hydrochloride which can form highly reactive compounds when combined with bleach (sodium hypochlorite). so DO NOT add bleach or acidic solutions directly to the sample preparation waste.

Publication protocol

Kit selection and DNA extraction
DNA extraction was performed using four commercial kits following the manufacturer's instructions. The kits, which used silica spin filter technology, were chosen for their easy and fast extraction methods. The following kits were evaluated: PowerSoil® DNA isolation and PowerFecal® kits (Mo Bio Laboratories Inc., Carlsbad, CA), QIAamp® DNA stool mini kit (Qiagen Inc., Valencia, CA), and NucleoSpin® Soil (Macherey-Nagel, Düren, Germany). For NucleoSpin® Soil, sample lysis was performed with the optional enhancer SX solution, as recommended by the manufacturer. In the rest of this article, the four kits are referred as PS, PF, QI, and NS, respectively.

To enable direct comparison of the kits, the same amount of starting material was used. DNA was extracted either from 250 mg of raw manure or from 250 mg of pellet after centrifugation of 90 mL of lagoon effluent at 16,000g for 30 min.

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Discussion

Discussion

2 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

2 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus using NucleoSpin® Soil from Macherey Nagel.

Paper title
Comparison of DNA extraction kits and modification of DNA elution procedure for the quantitation of subdominant bacteria from piggery effluents with real-time PCR
Full paper
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Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® Soil below.

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