Naive Pan T Cell Isolation Kit, human

Cell Isolation Naive Pan T cell

Experiment
Cell Isolation Naive Pan T cell
Product
Naive Pan T Cell Isolation Kit, human from Miltenyibiotec
Manufacturer
Miltenyibiotec

Protocol tips

Protocol tips
T cells were purified by magnetic bead selection using the Miltenyi pan-T cell isolation kit (Miltenyi Biotec, Auburn, CA). CLA− and CLA+ subsets were isolated by subsequent incubation of T cells in biotin anti-human CLA (BD Pharmingen) followed by anti-biotin microbeads and magnetic separation (Miltenyi).
Downstream tips
Positively selected cells were collected as the CLA+-enriched fraction and negatively selected cells were collected as the CLA− subset.

Publication protocol

Peripheral blood mononuclear cells were obtained as discarded pheresis material from red blood cell donors. T cells were purified by magnetic bead selection using the Miltenyi pan-T cell isolation kit (Miltenyi Biotec, Auburn, CA). CLA− and CLA+ subsets were isolated by subsequent incubation of T cells in biotin anti-human CLA (BD Pharmingen) followed by anti-biotin microbeads and magnetic separation (Miltenyi). Positively selected cells were collected as the CLA+-enriched fraction and negatively selected cells were collected as the CLA− subset. CLA− memory and naïve cells were then separated by use of anti-CD45RO microbeads and magnetic separation (Miltenyi).

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