|cells were counted, centrifuged at 300 g for 5 min at 4°C.|
|Pellet was resuspended in EasySep buffer (Catalog #20144) and incubated with DNase I solution (Catalog #07900) at a concentration of 100 μg/ml at room temperature for 15 min prior to labelling and separation. Following incubation, any cell aggregates were filtered out using 37 μm cell strainer (Catalog #27250). Cells were then diluted to a cencentration of 5 × 107 cells/ml in EasySep buffer. Sample was transferred to 5 ml polystyrene round-bottom tube (Catalog #38007) and isolation cocktail was added at a concentration of 50 μl/ml.|
|Sample was mixed and incubated at room temperature for 5 min. RAPIDSPHERES were thoroughly vortexed and added to the sample at a concentration of 40 μl/ml. Following this, the cell solution was topped up to 2.5 ml with EasySep buffer, mixed and placed on the EasySep magnet (Catalog #18000) for 3 min at room temperature.|
EasySep™ Human T Cell Isolation Kit from STEMCELL technologies has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Check out relevant papers found by Labettor's AI that are relevant for performing Cell Isolation Human T cells using EasySep™ Human T Cell Isolation Kit from STEMCELL technologies.
Download the product protocol from STEMCELL technologies for EasySep™ Human T Cell Isolation Kit below.Download manufacturer protocol
Check out videos that might be relevant for performing Cell Isolation Human T cells using EasySep™ Human T Cell Isolation Kit from STEMCELL technologies. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.
Fill out your contact details and receive price quotes in your InboxOutsource experiment