QIAamp DNA Stool Mini Kit

DNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus

Experiment
DNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus
Product
QIAamp DNA Stool Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- When using frozen stool samples, take care that the samples do not thaw until Buffer ASL is added in step 2 to lyse the sample; otherwise the DNA in the
sample may degrade.

Publication protocol

Kit selection and DNA extraction
DNA extraction was performed using four commercial kits following the manufacturer's instructions. The kits, which used silica spin filter technology, were chosen for their easy and fast extraction methods. The following kits were evaluated: PowerSoil® DNA isolation and PowerFecal® kits (Mo Bio Laboratories Inc., Carlsbad, CA), QIAamp® DNA stool mini kit (Qiagen Inc., Valencia, CA), and NucleoSpin® Soil (Macherey-Nagel, Düren, Germany). For NucleoSpin® Soil, sample lysis was performed with the optional enhancer SX solution, as recommended by the manufacturer. In the rest of this article, the four kits are referred as PS, PF, QI, and NS, respectively.

To enable direct comparison of the kits, the same amount of starting material was used. DNA was extracted either from 250 mg of raw manure or from 250 mg of pellet after centrifugation of 90 mL of lagoon effluent at 16,000g for 30 min.

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Discussion

Discussion

3 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

3 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus using QIAamp DNA Stool Mini Kit from Qiagen.

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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Stool Mini Kit below.

Download PDF Download manufacturer protocol

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