B Cell Isolation Kit II, human

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	During positive selection, the cell type of interest (in this case B-cells) are specifically targeted, labelled and actively magnetically removed from the sample by retention in the column in the presence of a magnetic field. These cells are then retrieved by plunging the column in the absence of a magnetic field.

Protocol tips
During positive selection, the cell type of interest (in this case B-cells) are specifically targeted, labelled and actively magnetically removed from the sample by retention in the column in the presence of a magnetic field. These cells are then retrieved by plunging the column in the absence of a magnetic field.

Publication protocol

"Isolation of B-cells from mononuclear cells using the Miltenyi cell isolation kits
Miltenyi isolation kits are based on a simple process, in which biotin conjugated antibody-labelled mononuclear cells are separated from unlabeled cells via a column in the presence of a magnetic field. During negative selection, the cell type of interest (in this case B-cells) remains unlabeled/“untouched” and in its native state, while the remaining unwanted mononuclear cells (in this case T-cells, NK cells and monocytes) are targeted via biotin labelled-antibodies specific for cell surface receptor(s) of those cell types. During positive selection, the cell type of interest (in this case B-cells) are specifically targeted, labelled and actively magnetically removed from the sample by retention in the column in the presence of a magnetic field. These cells are then retrieved by plunging the column in the absence of a magnetic field.

The efficiency of various Miltenyi isolation kits were tested. Specifically, 3 negative B-cell isolation kits, 1 positive B-cell isolation kit and 1 T-cell isolation kit were examined (see list below). All kits were operated according to the manufacturer’s instructions. For a more detailed description regarding this process refer to dx.doi.org/10.17504/protocols.io.yfzftp6 [PROTOCOL DOI] List of Miltenyi isolation kits examined
Isolation of B-cells from mononuclear cells by negative selection using the Miltenyi B-cell Isolation kit II (For detailed description of kit components see Datafile A in S1 File)

Isolation of B-cells from mononuclear cells by negative selection using the Miltenyi Naïve B-cell Isolation kit (For detailed description of kit components see Datafile D in S1 File)

Isolation of B-cells from mononuclear cells by negative selection using the Miltenyi CD43 Microbeads kit (For detailed description of kit components see Datafile E in S1 File)

Isolation of B-cells from mononuclear cells by positive selection using the Miltenyi CD19 positive Isolation kit (For detailed description of kit components see Datafile F in S1 File)"

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