MagCellect Human B Cell Isolation Kit

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	purified B cells (>95%) were isolated using MagCellect Human B Cell Isolation Kit (R&D systems, USA). B cell purity was assessed by flow cytometry using APC labeled CD19 antibody (Biolegend, USA).

Protocol tips
purified B cells (>95%) were isolated using MagCellect Human B Cell Isolation Kit (R&D systems, USA). B cell purity was assessed by flow cytometry using APC labeled CD19 antibody (Biolegend, USA).

Publication protocol

Blood samples were collected in heparinized tubes, and were diluted 1:1 with serum-free RPMI 1640 (Gibco, USA) medium. Peripheral blood mononuclear cells (PBMCs) were separated by Ficoll (GE Healthcare, USA) density gradient centrifugation. For RNA isolation and Western blotting, purified B cells (>95%) were isolated using MagCellect Human B Cell Isolation Kit (R&D systems, USA). B cell purity was assessed by flow cytometry using APC labeled CD19 antibody (Biolegend, USA). For transfection and cell culture, PBMCs were obtained from healthy volunteers.

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