MojoSort™ Human B Cell (CD43-) Isolation Kit

Cell Isolation B cell

Experiment
Cell Isolation B cell
Product
MojoSort™ Human B Cell (CD43-) Isolation Kit from BioLegend
Manufacturer
BioLegend

Protocol tips

Protocol tips
Human CD43− resting B cells were isolated from PBMCs using MojoSort Human B Cell (CD43−) Isolation Kit (BioLegend), according to the manufacturer's protocols.
Downstream tips
Isolated resting B cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 10 mM HEPES, 100 μg/ml streptomycin, 100 U/ml penicillin, and 1 mM 2-mercaptoethanol.

Publication protocol

Human PBMCs were separated by density gradient centrifugation using Lympholyte-H (Cedarlane, Burlington, Canada). Human CD43− resting B cells were isolated from PBMCs using MojoSort Human B Cell (CD43−) Isolation Kit (BioLegend), according to the manufacturer's protocols. Isolated resting B cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 10 mM HEPES, 100 μg/ml streptomycin, 100 U/ml penicillin, and 1 mM 2-mercaptoethanol. For some experiments, resting B cells were stimulated with 1 μg/ml anti-human IgM Ab (BioLegend), 1 μg/ml recombinant human CD40L (BioLegend), 100 μg/ml poly(I:C), and/or 50 μg/ml imiquimod (R837; InvivoGen, San Diego, CA, USA). After incubation for 24 h, the cells were immediately used for flow cytometric analyses, and the supernatants were stocked at – 80°C until use.

Full paper   Login or join for free to view the full paper.

Reviews

MojoSort™ Human B Cell (CD43-) Isolation Kit from BioLegend has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell Isolation B cell using MojoSort™ Human B Cell (CD43-) Isolation Kit from BioLegend.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from BioLegend for MojoSort™ Human B Cell (CD43-) Isolation Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell Isolation B cell using MojoSort™ Human B Cell (CD43-) Isolation Kit from BioLegend. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms