QuantiTect Multiplex PCR Kit (1000)

PCR Multiplex PCR - Bacterial DNA

Experiment
PCR Multiplex PCR - Bacterial DNA
Product
QuantiTect Multiplex PCR Kit (1000) from Qiagen
Manufacturer
Qiagen

Protocol tips

Publication protocol

The EDSP’s Salmonella/Shigella/Campylobacter real-time, multiplex PCR assay was based on that published by Wiemer, D et al. but modified to exclude the Yersinia primer/probe set and include the exogenous, spike-in green fluorescent protein (GFP, IDT) IAC.5 Total reaction volumes of 25-μL contained Qiagen QuantiTect Multiplex PCR Kit (with ROX dye) reagents, with final concentrations as follows: 0.4 μM each primer and 0.2 μM each probe with the exception of the GFP probe, which was 0.04 μM; 0.1 nanograms GFP DNA; and 0.5-2.0 μg (2.5 μL) sample DNA. The thermal cycling program was as follows: 1 cycle of 15 min at 95 °C; 45 cycles of 1 min at 94 °C, 30 sec at 50 °C, and 30 sec at 72 °C; and 1 cycle of 10 min at 72 °C.

Full paper   Login or join for free to view the full paper.

Reviews

QuantiTect Multiplex PCR Kit (1000) from Qiagen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: R. Verma India

Multiplex PCR and qPCR

I have been doing multiplex PCR and qPCR separately. Is there a way to combine them in a single reaction using Sybr Green?

Discussion

5 years ago

Author: Roland Lafontaine France

My multiplex PCR has decreased in sensitivity

Hello there! My multiplex PCR has decreased in sensitivity even though I have not changed any of the parameters. The materials are in good condition and the PCR machine is well calibrated. I have tested multiple samples but one of my targets has decreased in sensitivity.

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing PCR Multiplex PCR - Bacterial DNA using QuantiTect Multiplex PCR Kit (1000) from Qiagen.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Qiagen for QuantiTect Multiplex PCR Kit (1000) below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing PCR Multiplex PCR - Bacterial DNA using QuantiTect Multiplex PCR Kit (1000) from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms