QIAquick 96 PCR BioRobot Kit (4)

DNA gel extraction / PCR product purification Product size < 15Kb

Experiment
DNA gel extraction / PCR product purification Product size < 15Kb
Product
QIAquick 96 PCR BioRobot Kit (4) from Qiagen
Manufacturer
Qiagen

Protocol tips

Publication protocol

Microarray assay: Defined 200- to 400-bp fragments of selected cDNAs (38) were generated by RT-PCR (Superscript II, Invitrogen, Groningen, The Netherlands) and were cloned into pGEM-T Vector (Promega, Mannheim, Germany), and the sequence was verified. Inserts were amplified by using vector sequence-derived primers with the sense primer carrying a 5'-amino modification. Amplified inserts were purified (Qiaquick 96 PCR BioRobot Kit, QIAGEN, Hilden, Germany), checked on an agarose gel, diluted to a concentration of 100 ng/μl, and finally spotted four times on treated glass slides with a customized ink jet spotter.

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Manufacturer protocol

Download the product protocol from Qiagen for QIAquick 96 PCR BioRobot Kit (4) below.

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