DNeasy UltraClean Microbial Kit

DNA isolation / purification Yeast - Saccharomyces cerevisiae

Experiment
DNA isolation / purification Yeast - Saccharomyces cerevisiae
Product
DNeasy UltraClean Microbial Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
DO NOT add bleach or acidic solutions directly to the sample-preparation
waste.
Downstream tips
- Include RNAse treatment for 15-20 min.
- Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA isolation
Genomic DNA was isolated from single colonies using the commercial kit UltraClean™ Microbial DNA Isolation Kit (MoBio, USA) according to the manufacturer's protocol.

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Reviews

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Discussion

Discussion

4 years ago

Author: France

How to avoid genomic DNA contamination when isolating mitochondrial DNA?

Greetings! I am trying to isolate mitochondrial DNA from S. cerevisiae while avoiding contamination from genomic DNA. Any and all help is greatly appreciated.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Yeast - Saccharomyces cerevisiae using DNeasy UltraClean Microbial Kit from Qiagen.

Paper title
Isolation, identification and characterization of regional indigenous strains
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for DNeasy UltraClean Microbial Kit below.

Download PDF Download manufacturer protocol

Videos

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