Penta·His HRP Conjugate Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Follow manufacturer’s instructions

Protocol tips
Follow manufacturer’s instructions

Publication protocol

Cultures of selected clones were grown overnight in 10 mL of Luria-Bertani broth supplemented with 0.15 mM KNO3 and 100 μg/mL ampicillin. High aeration samples were diluted 1:100 into 50 mL fresh media in a 250 mL baffled flask and allowed to grow to an OD600 of ∼0.5. Protein expression was induced with 1 μg/mL mitomycin C and grown overnight at 30°C shaking at 150 RPM. Low aeration samples were diluted 1:24 in 1 mL of fresh media contained in individual wells of a 96-well culture box (Axygen Scientfic), and grown to an OD600 of ∼0.5. Protein expression was induced with 1 μg/mL mitomycin C and grown overnight at 30°C shaking at 150 RPM on a microtiter plate shaker.
After 18 hours of induced growth, cultures were serially diluted and plated on LB plates supplemented with 100 μg/mL ampicillin. Plates were incubated overnight at 37°C, and colonies were counted to enable normalized loading and separation of cellular lysate using SDS-PAGE. Detection of expressed protein was done using the Penta-His HRP Conjugate Kit and following the manufacturer's provided protocol (Qiagen).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein tag Detection of His-tagged proteins using Penta·His HRP Conjugate Kit from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for Penta·His HRP Conjugate Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Protein tag Detection of His-tagged proteins using Penta·His HRP Conjugate Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.