QIAamp DNA Blood BioRobot MDx Kit (12)
DNA isolation / purification Tissue - blood / plasma
Publication protocol
Illumina infinium human methylation 450K beadchip: Blood samples were obtained from each participant via venipuncture and sent to Roswell Park Cancer Institute for DNA extraction. Genomic DNA was isolated from 0.3 ml of whole blood using the Qiagen BioRobot Universal System and the QIAamp DNA blood BioRobot MDx Kit (Qiagen, Valencia, CA, USA) following the manufacturer’s recommended protocol. DNA methylation profiling was performed by Roswell Park Cancer Institute using the Human Methylation 450 K BeadChip (Illumina, San Diego, CA, USA). DNA extraction and methylation profiling were done blinded to group assignment. Five hundred nanogram of high-quality genomic DNA measured by picogreen quantitation (Life technologies, Grand Island, NY, USA) was bisulphite converted, amplified, fragmented and hybridized to the Illumina Infinium Human Methylation 450 K Beadchip using standard Illumina protocol. Data were processed using Illumina's GenomeStudio methylation module (v1.9.0).Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
What DNA isolation kit would work for insect samples?
Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?
Papers
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Manufacturer protocol
Download the product protocol from Qiagen for QIAamp DNA Blood BioRobot MDx Kit (12) below.
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