PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit

DNA isolation / purification Yeast - Cryptococcus neoformans

Experiment
DNA isolation / purification Yeast - Cryptococcus neoformans
Product
PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
Do not use bleach to clean the inside of the PowerVac™ Manifold or to rinse the PowerVac™ Mini Spin Filter Adapters when attached to the manifold.

Publication protocol

DNA extraction
Four extraction techniques were performed in triplicate on the 200 μL aliquots of both the pooled plaque and pooled saliva homogenates. Three commonly used commercial DNA extraction kits were used as per manufacturer’s instructions, namely the MoBio PowerSoil DNA Isolation Kit (M), Qiagen QIAamp DNA Mini Kit (Q) and the Zymo Bacterial/Fungal DNA Mini Prep (Z) (). Additionally, a previously described phenol:chloroform-based method for DNA isolation from saliva was used (P) []. Mechanical cell rupture was performed using a Qiagen TissueLyser II at 30 Hz for 2 x 50 s, as applicable. For each method, an extraction blank (PCR-grade water) was used to ascertain potential kit and/or reagent contamination.

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Discussion

Discussion

5 years ago

Author: France

How to avoid genomic DNA contamination when isolating mitochondrial DNA?

Greetings! I am trying to isolate mitochondrial DNA from S. cerevisiae while avoiding contamination from genomic DNA. Any and all help is greatly appreciated.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Yeast - Cryptococcus neoformans using PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit from Qiagen.

Paper title
Evaluating the Impact of DNA Extraction Method on the Representation of Human Oral Bacterial and Fungal Communities
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit below.

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