Gentra Puregene Yeast/Bact. Kit

DNA isolation / purification Yeast - Candida parapsilosis

Experiment
DNA isolation / purification Yeast - Candida parapsilosis
Product
Gentra Puregene Yeast/Bact. Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
Frozen yeast/bacterial samples should be thawed and equilibrated to room temperature (15–25°C) before beginning the procedure.

Publication protocol

Template DNA preparation and multiplex PCR assay
The genomic DNA from reference strains and clinical isolates of different species was extracted by using Gentra Puregene Yeast DNA extraction kit (Qiagen, Hilden Germany) according to kit instructions or by the rapid method using Chelex-100. A loop full of yeast colony grown on Sabouraud dextrose agar plate was suspended in 1 ml of sterile water in a microcentrifuge tube containing 50 mg Chelex-100 (Sigma-Aldrich Co., St. Louis, MO, USA), the contents were heated at 95°C for 20 min and then centrifuged. The supernatant was transferred to a new tube and typically 2 μl was used for mPCR.

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Discussion

Discussion

4 years ago

Author: France

How to avoid genomic DNA contamination when isolating mitochondrial DNA?

Greetings! I am trying to isolate mitochondrial DNA from S. cerevisiae while avoiding contamination from genomic DNA. Any and all help is greatly appreciated.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Yeast - Candida parapsilosis using Gentra Puregene Yeast/Bact. Kit from Qiagen.

Paper title
Simple, Low-Cost Detection of Complex Isolates and Molecular Fingerprinting of Strains in Kuwait by ITS Region Sequencing and Amplified Fragment Length Polymorphism Analysis
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Manufacturer protocol

Download the product protocol from Qiagen for Gentra Puregene Yeast/Bact. Kit below.

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