EZ1 DSP DNA Blood Kit (48)

DNA isolation / purification Tissue - blood / plasma

Experiment
DNA isolation / purification Tissue - blood / plasma
Product
EZ1 DSP DNA Blood Kit (48) from Qiagen
Manufacturer
Qiagen

Protocol tips

Publication protocol

Genomic DNA (gDNA) was isolated from 350 µL EDTA patient blood using QIAGEN EZ1 DSP DNA Blood kit (QIAGEN) on QIAGEN EZ1 Advanced XL system.6 The extracted gDNA sample was quantified with Quant‐iT™ dsDNA PicoGreen™ Assay Kit (Thermo Fisher) using SpectraMax® Gemini EM Microplate Spectrofluorometer (Molecular Devices). The integrity of gDNA was confirmed by running 0.8% E‐gel (Thermo Fisher). A DNA library construction started with fragmentation using Covaris Sonicator E220 (Covaris) to shear 1 µg gDNA to the fragments size of 300‐500 base pairs (bp). A fragmented DNA sample was then treated with Illumina TruSeq®DNA PCR‐Free Library Prep Kits in order to yield a final library, in which fragments were ligated with a molecular barcoded adapter. The ligated fragment size and concentration of a DNA library sample was measured using Fragment Analyzer™ (Advanced Analytical) and KAPA Library Quantification Kits on Roche LightCycler® 480 Instrument (Roche). A library with the concentration more than 3 nmol/L (300‐600 bp) was passed for sequencing on Novaseq 6000 S2 Flow Cell (FC).

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Discussion

Discussion

5 years ago

Author: Denmark

What DNA isolation kit would work for insect samples?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for EZ1 DSP DNA Blood Kit (48) below.

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