RNeasy 96 Universal Tissue Kit (4)

RNA isolation / purification Tissue - Mouse Kidney

Experiment
RNA isolation / purification Tissue - Mouse Kidney
Product
RNeasy 96 Universal Tissue Kit (4) from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
Follow manufacturer's instructions

Publication protocol

mRNA Analyses: For mRNA analysis, fresh renal cortex samples were collected from UUO studies using a 2-mm-diameter biopsy punch (Integra, Plains- boro, NJ) and placed in RNALater solution (Life Technologies, Carls- bad, CA). Total RNA was isolated from homogenized cortex punches using the Qiagen RNeasy-96 Universal Tissue Kit (Qiagen, Valencia, CA) following the manufacturer’s protocol. Reverse transcription of RNA to cDNA was done using Bio-Rad iScript cDNA Synthesis kits (Bio-Rad, Hercules, CA). All genes of interest were targeted with TaqMan Assays-on-Demand Gene Expression primers with FAM dye-labeled MGB probes from Applied Biosystems (Foster City, CA). TaqMan Universal Master Mix II with UNG (Applied Biosys- tems) was used in PCR reactions. Real time PCR was performed using the Bio-Rad CFX384 Touch system (Bio-Rad).

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

4 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy 96 Universal Tissue Kit (4) below.

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