MasterPureTM-Yeast DNA Purification kit

DNA isolation / purification Yeast - Candida albicans

Experiment
DNA isolation / purification Yeast - Candida albicans
Product
MasterPureTM-Yeast DNA Purification kit from Epicentre
Manufacturer
Epicentre

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA Extraction
After paraffin removal using octane, DNA was extracted using the MasterPure™ Yeast DNA Purification Kit (Epicentre® Biotechnologies, Madison, WI) with an additional bead beating step to optimise fungal cell lysis as described previously [6]. This kit utilizes a non enzymatic approach to lyse fungal cell walls at 65°C. Contaminating macromolecules were then removed by a precipitation step, followed by DNA precipitation with isopropanol for purification of DNA. Extracted DNA was dissolved in 75 μl of 0,5% Triton X and stored at −20°C until PCR testing.

In order to evaluate the effect of thermal energy on the amount of amplifiable DNA, two modifications of the previously described protocol were studied. First, the initial incubation step in lysis buffer was carried out at either 65°C, as proposed by the manufacturer, or at 90°C for one hour with aliquots of all samples. Second, the duration of incubation at 90°C was increased from one hour to three, six and twelve hours using the samples 1,4,6 and 7 in duplicate extractions.

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Discussion

Discussion

2 years ago

Author: France

How to avoid genomic DNA contamination when isolating mitochondrial DNA?

Greetings! I am trying to isolate mitochondrial DNA from S. cerevisiae while avoiding contamination from genomic DNA. Any and all help is greatly appreciated.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Yeast - Candida albicans using MasterPureTM-Yeast DNA Purification kit from Epicentre.

Paper title
Enhanced fungal DNA-Extraction from Formalin fixed, paraffin embedded tissue specimens by application of thermal energy
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Manufacturer protocol

Download the product protocol from Epicentre for MasterPureTM-Yeast DNA Purification kit below.

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