Wizard® Genomic DNA Purification Kit

DNA isolation / purification Cells - primary cells human primary keratinocytes

Experiment
DNA isolation / purification Cells - primary cells human primary keratinocytes
Product
Wizard® Genomic DNA Purification Kit from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Publication protocol

Cell Compatibility of Photopolymerization Process
To verify the cell compatibility of the BioInk polymerization process, a cyclobutane pyrimidine dimer (CPD) enzyme-linked immunosorbent assay (ELISA) was performed. CPD formation is one of the predominant types of DNA damage caused by UV light. Dermis models were printed, and genomic DNA was isolated immediately after printing (day 0) and after 5 days of culture with the Wizard Genomic DNA Purification Kit (Promega, Fitchburg, WI) according to the manufacturer’s protocol. Each DNA sample (n = 3) was obtained from the processing of three different and pooled dermal models. Fibroblast DNA content was measured at 260 nm with a NanoDrop (Fisher Scientific, Hampton, NH). As blanks, 3D models were produced by replacing cell suspension with sterile water. In parallel, genomic DNA was isolated from fibroblasts cultured in 2D with and without UV exposure for 10 min at an intensity of 5 mW/cm2 (λ = 260 nm) to obtain positive and negative controls, respectively. The CPD detection was performed with the OxiSelect UV-Induced DNA Damage ELISA Kit (Cell Biolabs, San Diego, CA) following the manufacturer’s guidelines. The sample absorption was measured at 485 nm with a microplate reader (FLUOstar OPTIMA; BMG Labtech, Ortenberg, Germany). The blank absorption values were subtracted from the cell samples’ absorption values.

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Discussion

Discussion

1 year ago

Author: R. Verma India

DNA isolation column clogged

During centrifugation, the column got clogged and I was unable to continue with the protocol. How can I unclog it?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Cells - primary cells human primary keratinocytes using Wizard® Genomic DNA Purification Kit from Promega.

Paper title
Standardized 3D Bioprinting of Soft Tissue Models with Human Primary Cells.
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Manufacturer protocol

Download the product protocol from Promega for Wizard® Genomic DNA Purification Kit below.

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