DNeasy UltraClean Microbial Kit

DNA isolation / purification Bacteria - Gram positive Enterococcus faecium

Experiment
DNA isolation / purification Bacteria - Gram positive Enterococcus faecium
Product
DNeasy UltraClean Microbial Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

Enterococcal strains were cultured overnight in BHI broth prior to genomic DNA (gDNA) extraction. Extraction was performed using a Qiagen blood and tissue DNeasy kit using a previously reported protocol (38). To isolate E. coli gDNA, bacteria were grown overnight in LB prior to extraction using either the blood and tissue DNeasy kit (Qiagen) or the UltraClean microbial DNA isolation kit (Qiagen) according to the manufacturer’s instructions. Whole-genome amplification (WGA) control DNA was generated by amplification of native gDNA using the REPLI-g kit (Qiagen) according to the manufacturer’s instructions.

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Reviews

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Discussion

Discussion

4 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

4 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram positive Enterococcus faecium using DNeasy UltraClean Microbial Kit from Qiagen.

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Manufacturer protocol

Download the product protocol from Qiagen for DNeasy UltraClean Microbial Kit below.

Download PDF Download manufacturer protocol

Videos

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