MagNA Pure Compact Nucleic Acid Isolation Kit I

DNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

Experiment
DNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa
Product
MagNA Pure Compact Nucleic Acid Isolation Kit I from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Upstream tips
For isolation of nucleic acids from relatively large organisms
such as Mycobacterium, it is important to transfer the supernatant before the cells settle to the bottom of the tube.

Publication protocol

Staphylococcus epidermidis, Streptococcus mitis, Propionibacterium acnes, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger and Fusarium solani were mixed with an internal control virus and treated with: 1) proteinase K; 2) lyticase and 3) lyticase followed by proteinase K. DNAs was manually extracted using the QIAmp DNA Mini kit or the MagNA Pure Compact automate. DNA extraction yields and the inhibitors were assessed with a phocid Herpesvirus. Bacterial detection was performed using TaqMan real-time PCR and yeasts and filamentous Fungi with HRM (real-time PCR followed by high-resolution melting analysis).


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Reviews

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Discussion

Discussion

2 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

2 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa using MagNA Pure Compact Nucleic Acid Isolation Kit I from Roche Lifesciences.

Paper title
Enzymatic Treatment of Specimens before DNA Extraction Directly Influences Molecular Detection of Infectious Agents
Full paper
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Manufacturer protocol

Download the product protocol from Roche Lifesciences for MagNA Pure Compact Nucleic Acid Isolation Kit I below.

Download PDF Download manufacturer protocol

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