PureLink Genomic DNA Mini Kit

DNA isolation / purification Tissue - lung

Experiment

DNA isolation / purification Tissue - lung

Product

PureLink Genomic DNA Mini Kit from Thermo Fisher Scientific

Manufacturer

Thermo Fisher Scientific

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

2.2.3. DNA quantification
Isolation of DNA from tracheal samples was performed using a PureLink DNA extraction kit (Invitrogen) according to the manufacturer’s instructions. Briefly, samples were digested overnight in proteinase K and DNA was isolated using the supplied buffers and mini-columns. Quantification of eluted DNA was carried out by spectrophotometry using a NanoDrop 8000 (Thermo Scientific). All samples had a 260/280 ratio of between 1.8 and 2.0.

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Reviews

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Discussion

4 years ago

Author: Denmark

What DNA isolation kit would work for insect samples?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Tissue - lung using PureLink Genomic DNA Mini Kit from Thermo Fisher Scientific.

Paper title

Vacuum-assisted decellularization: an accelerated protocol to generate tissue-engineered human tracheal scaffolds

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PureLink Genomic DNA Mini Kit below.

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Videos

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