GFP-CERTIFIED® Apoptosis/Necrosis detection kit

Necrosis MDA-MB-231

Experiment
Necrosis MDA-MB-231
Product
GFP-CERTIFIED® Apoptosis/Necrosis detection kit from Enzo Life Sciences
Manufacturer
Enzo Life Sciences

Protocol tips

Upstream tips
• This kit contained 7-amino-actinomycin D (7-AAD) and Annexin V, which were used to detect necroptosis and apoptosis, respectively.
Protocol tips
• Cells were observed under a confocal microscope (×200) after staining with 7-aminoactinomycin D (7-AAD) to identify necroptotic cells coated in red color.

Publication protocol

Necroptosis was also analyzed using a Green Fluorescent Protein-Certified Apoptosis/Necrosis Detection kit (cat. No. ENZ-51002; Enzo Life Sciences), according to the manufacturer’s protocol. This kit contained 7-aminoactinomycin D (7-AAD) and Annexin V for the detection of necroptosis and apoptosis, respectively. In brief, cells were plated into 8-well chamber slides for 24 h of serum starvation. Cells were observed under confocal microscope (×200) after staining with 7-aminoactinomycin D (7-AAD) to identify necroptotic cells coated in red color and Annexin V-EnzoGold (enhanced Cyanine-3) to detect apoptotic cells stained yellow color.

Full paper   Login or join for free to view the full paper.

Reviews

GFP-CERTIFIED® Apoptosis/Necrosis detection kit from Enzo Life Sciences has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Necrosis MDA-MB-231 using GFP-CERTIFIED® Apoptosis/Necrosis detection kit from Enzo Life Sciences.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Enzo Life Sciences for GFP-CERTIFIED® Apoptosis/Necrosis detection kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Necrosis MDA-MB-231 using GFP-CERTIFIED® Apoptosis/Necrosis detection kit from Enzo Life Sciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms