MessageAmp II aRNA Amplification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Check the cDNA Binding Buffer for precipitation before using it. If a precipitate is visible, redissolve it by warming the solution to 37°C for up to 10 min and vortexing vigorously. Cool to room temperature before use

Protocol tips
Check the cDNA Binding Buffer for precipitation before using it. If a precipitate is visible, redissolve it by warming the solution to 37°C for up to 10 min and vortexing vigorously. Cool to room temperature before use

Publication protocol

mRNA profiling
Prenatal samples passing RNA quality control (QC) were amplified and profiled. Briefly, samples were amplified and labeled using a custom 2 cycle Ultra Low Input procedure, using components of MessageAmp II aRNA Amplification kit (AM1751) for the first amplification cycle (using oligodT), and components of MessageAmp II Biotin Enhanced Single Round aRNA Amplification kit (AM1791) for the second amplification cycle (using both random hexamers and oligodT). For prenatal samples, 2ng of total RNA was added to the initial reaction mix together with 250ng of pBR322 (Invitrogen). Following the first cycle of IVT, the plasmid carrier was removed with a DNaseI (Qiagen) treatment. The first cycle IVT products were purified using the QiagenMinElute Kit (Qiagen). Input into the second cycle was normalized to 400ng. Hybridization was to catalog GeneChip Rhesus Macaque Genome Arrays from Affymetrix containing 52,803 probe sets/sequences.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for MessageAmp II aRNA Amplification Kit below.

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