Lipofectamine® 2000 Transfection Reagent

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	Do not add antibiotics to the Transfection Medium

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

Transfection Efficiency Assay PANC-1 cells were seeded
in  six-well  plates  and  maintained  with  complete  medium  containing no antibiotics. Transfection was performed when cell  confluence  reached  approximately  80%.  First,  a  series of  complexes  was  prepared  by  thoroughly  mixing  100 pmol  of  FAM-labeled  siRNA  with  the  corresponding  amounts  of 
mPEG–SeSe–PEI, PEG–PEI, PEI, and Lipo2000 and then diluted with Opti-MEM to a specific volume before being added to the wells. After being exposed to these different complexes for 5 h, the PANC-1 cells were harvested. FCM was performed with  BD  FACS  Canto-II  (Becton,  Dickinson  and  Company, Franklin  Lakes,  NJ,  U.S.A.)  to  determine  the  transfection  efficiency,  and  the  data  were  processed  with  FlowJo  software, version  7.6  (FlowJo,  Ashland,  OR,  U.S.A.).  Phosphate  buffered  saline  (PBS)/siRNA  served  as  the  negative  control,  and Lipo2000/siRNA  (a mixture  of  7.5 µL Lipo2000 and 100 pmol FAM-labeled siRNA) was used as the positive control

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® 2000 Transfection Reagent below.

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