jetPEI® DNA transfection, HTS application

DNA transfection Mammalian cells - Immortalized cell lines SMMC-7721

Experiment
DNA transfection Mammalian cells - Immortalized cell lines SMMC-7721
Product
jetPEI® DNA transfection, HTS application from Polyplus transfections
Manufacturer
Polyplus transfections

Protocol tips

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

ARE luciferase reporter vector, siRNA, and vector of Nrf2 and Keap1 were designed and purchased from GeneChem (NM 001145413). siNrf2, 5′-GGCATTTCACTAAACACAA-3′; siKeap1, 5′-CTTAATTCAGCTGAGTGTT-3′. The vectors were transfected into HCC cells with jetPEI DNA transfection reagent (Polyplus). Nrf2 cDNA was cloned from the whole cDNA synthesized by total RNA and constructed into plenti-EF3.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA transfection Mammalian cells - Immortalized cell lines SMMC-7721 using jetPEI® DNA transfection, HTS application from Polyplus transfections.

Paper title
Gankyrin has an antioxidative role through the feedback regulation of Nrf2 in hepatocellular carcinoma
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Manufacturer protocol

Download the product protocol from Polyplus transfections for jetPEI® DNA transfection, HTS application below.

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