Xfect™ Transfection Reagent

Protocol tips

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XFect–DNA complex formation should be performed in the absence of serum, antibiotics, and proteins.

Upstream tips
XFect–DNA complex formation should be performed in the absence of serum, antibiotics, and proteins.

Publication protocol

3T3-L1 preadipocytes were cultured in high-glucose DMEM complete culture medium supplemented with 10% fetal bovine serum and antibiotics. At around 70–80% confluence, cells were transfected by Xfect Transfection Reagent (Clontech Laboratories) per the manufacturer's instructions. After 48 h culture and 3× PBS washes, the transfected medium and cell lysates were analyzed by Western blot.

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Manufacturer protocol

Download the product protocol from Takara Bio Inc for Xfect™ Transfection Reagent below.

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