Xfect™ Transfection Reagent

siRNA / RNAi /miRNA transfection Rat - H9c2 Cationic and neutral lipids

Experiment
siRNA / RNAi /miRNA transfection Rat - H9c2 Cationic and neutral lipids
Product
Xfect™ Transfection Reagent from Takara Bio Inc
Manufacturer
Takara Bio Inc

Protocol tips

Protocol tips
Always add your plasmid to the buffer before adding Xfect Polymer.

dilute 5 µg of your plasmid DNA with Xfect Reaction Buffer to a final volume of 100 µl.

Incubate for 10 min at room temperature.

Add the nanoparticle complex solution dropwise to the cell culture medium.

Incubate the plate at 37°C for 4 hr to overnight.

Publication protocol

SIRT1 small interfering RNA (siRNA) was synthesized by Genepharma Corp. (Shanghai, China). The sequences of mouse SIRT1 siRNA were: 5’-UUG CAGAAACAGUGAGAAA-3’. The negative control siRNA was from Genepharma. Transfection of siRNA was performed by using the Xfect siRNA transfection reagents (Takara, Japan).

Full paper   Login or join for free to view the full paper.

Reviews

Xfect™ Transfection Reagent from Takara Bio Inc has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / RNAi /miRNA transfection Rat - H9c2 Cationic and neutral lipids using Xfect™ Transfection Reagent from Takara Bio Inc.

Paper title
Salidroside Attenuates Ventilation Induced Lung Injury via SIRT1-Dependent Inhibition of NLRP3 Inflammasome.
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Takara Bio Inc for Xfect™ Transfection Reagent below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / RNAi /miRNA transfection Rat - H9c2 Cationic and neutral lipids using Xfect™ Transfection Reagent from Takara Bio Inc. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms