TurboFect Transfection Reagents

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Dilute 1µg of DNA in 100µL of serum-free DMEM or other serum-free growth medium. Incubate 15-20 minutes at room temperature. Add transfection reagent/DNA mixture to samples and incubate at 37°C in a CO2 incubator.	Analyse expression after 24-48 hours

Protocol tips
Dilute 1µg of DNA in 100µL of serum-free DMEM or other serum-free growth medium. Incubate 15-20 minutes at room temperature. Add transfection reagent/DNA mixture to samples and incubate at 37°C in a CO2 incubator.
Downstream tips
Analyse expression after 24-48 hours

Publication protocol

For reporter gene assays and protein overexpression, cells were transfected by use of TurboFect (Fisher Scientific) according to the recommended instructions. Small interfering RNA (siRNA) was transfected according to TurboFect recommendations. Cells were transfected for 3 h, followed by medium replacement, and were harvested or had the serum withdrawn 24 to 48 h later, unless otherwise indicated. Myc(N)-β-catenin was N-terminally truncated by 89 amino acids (aa) prior to tagging to prevent GSK3β phosphorylation and degradation, and the resulting construct was named β-catenin (act) (activated).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TurboFect Transfection Reagents below.

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