Lipofectamine® 2000 Transfection Reagent

siRNA / RNAi /miRNA transfection Rat - Ar4-2j (Pancreatic tumor) Lipid based

Experiment
siRNA / RNAi /miRNA transfection Rat - Ar4-2j (Pancreatic tumor) Lipid based
Product
Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Add 1:1 ratio of DNA to diluted
Lipofectamine® 2000 Reagent and Incubate for 5 minutes at room temperature.

Incubate cells for 1–3 days at 37°C. Then analyze transfected cells.

Publication protocol

For transfection, 4 × 104 HEK293 cells were seeded on 96-well plates 24 hours before transfection. A reporter construct containing the 3′UTR- of HNF6 downstream of the Renilla luciferase gene27 and the control firefly (pGL3-CMV) luciferase vector, 100 ng and 0.2 ng, respectively, were cotransfected with 25 nM control miRNA or miRIDIAN miR-Mimics (Dharmacon, ThermoFisher Scientific, Tournai, Belgium) and 1 μL Lipofectamine 2000 (Invitrogen). Luciferase activity was measured 24 hours after transfection using the Dual Luciferase reporter assay system (Promega, Leiden, Netherlands) and a Glomax96 luminometer (Promega).

Full paper   Login or join for free to view the full paper.

Reviews

Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / RNAi /miRNA transfection Rat - Ar4-2j (Pancreatic tumor) Lipid based using Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific.

Paper title
Let-7b and miR-495 stimulate differentiation and prevent metaplasia of pancreatic acinar cells by repressing HNF6.
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® 2000 Transfection Reagent below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / RNAi /miRNA transfection Rat - Ar4-2j (Pancreatic tumor) Lipid based using Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms