3 years ago
Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?
Experiment: DNA quantification Human - PC-3
The short answer is yes. If you intend to do a precise quantification you should use nuclease-free water in order to avoid having your DNA slightly degrade, which in turn will alter the DNA concentration. However, if you are using it for routine techniques and you measure the DNA reasonably quickly, using nuclease-free water should not matter that much.