2 years ago
Sander J. Mikkelsen2
What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?
HUVEC (human umbilical vein endothelial cells) are the endothelial cells isolated from the umbilical vein and usually supplied as a pool obtained from several human donors. They are considered a primary endothelial cell line as they are untransformed cells that can be maintained in culture for several passages without growth inhibition. They are often used for the angiogenesis studies not because of adequate representation of the vascular endothelial cells in vivo, but because they are relatively easy to obtain and culture. However, endothelial cells in vivo are heterogeneous with significant differences found between organs, microenvironment (e.g. quiescent vs tumor), large and small blood vessels, and arteries and veins thus depending on the actual scientific problem it might be better to use site specific endothelial cells instead HUVEC.