Discussion

2 years ago

When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

Experiment: Protein isolation Tissue - Mouse lung tissue

1 Comment


HIF proteins at the presence of atmospheric oxygen concentration have a half-life of about five minutes. This means that you should harvest your tissue samples within a couple of minutes otherwise the proteins will start degrading.

Answered 2 years ago


Can you help?

Start your discussion


Share your thoughts or question with experts in your field

Start discussion
Become shareholder Discussions About us Contact Privacy Terms