2 years ago
I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?
Experiment: Protein isolation Tissue - Human placental tissue
HIF proteins at the presence of atmospheric oxygen concentration have a half-life of about five minutes. This means that you should harvest your tissue samples within a couple of minutes otherwise the proteins will start degrading.
Answered 2 years ago
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