2 years ago
The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?
Experiment: DNA isolation / purification Bacteria - Gram positive Clostridium difficile
Interesting question indeed!
Answered 2 years ago
Dhr. L.S. Petitiaux
Start with more cells or run multiple purifications and combine the results. If this is plasmid DNA, be sure to choose a high copy form. Remember to rely heavily on cold precipitation temps as well. Follow the time events in your recipe accurately.
Answered 1 year ago
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